BSD BMD - Research Staff: Greene Lab
About the Department
Our major research focus is understanding the biology and therapeutic vulnerabilities of estrogen receptor positive breast cancer. We investigate the mechanisms of ER+ breast cancer prevention, progression, metastasis, and response to experimental therapies using cell based and in vivo breast cancer models, some of which are derived directly from patients. Additional interests included generating and testing organoid cultures derived from breast tumor tissue.
The job develops and implements programs to promote a research project or contributes to the scientific direction of a research resource.
A full-time research professional is needed to work with the PI to independently mentor and supervise postdoctoral scholars, graduate students and/or undergraduates with several ongoing projects that focus on the role of steroid receptors in cancer progression and pattern recognition receptors in innate immunity and inflammation. Our group works at the intersection of cell biology, assay-development, drug-discovery, and structural biology to understand therapeutic potentials of protein targets and provide novel insights into protein function and drug discovery. The ideal candidate has a proven track record of expressing and purifying recombinant proteins for structural biology and drug discovery applications as well as additional experience performing, developing, and optimizing in vitro and cell-based assays. This individual will be expected to carry out multiple tasks concurrently and independently and will need to have good organizational, supervisorial, as well as laboratory skills. The successful candidate will ensure compliance of research activities with institutional, state, and federal regulatory policies, procedures, directives, mandates, and writes articles, reports, grants, and manuscripts describing research findings and future directions.
- Maintaining mammalian cell culture and generating/selecting new cell lines using standard methods including lentiviral transduction and CRISPR knock out/knock.
- Use standard tools of molecular biology to generate protein expression constructs for downstream in vitro expression and cell biology experiments.
- Expression of target proteins using E.coli, yeast, SF9, and HEK293F systems.
- Purification of recombinant proteins using affinity, ion exchange, gel filtration, and other chromatography methods as implemented on an AKTA system.
- Characterization of recombinant proteins using biophysical tools including DSF, ITC, SPR, FP, SEC, CD, and ELISA for subsequent biophysical analysis.
- Use the tools of structural biology (xray crystallography and cryo EM) to understand and visualize the mechanisms of small molecule binding to protein targets of interest.
- Develops and executes target specific enzyme-activity and cell-based assays (including high-throughput assays) to elucidate protein function and identify small molecule inhibitors, nanoparticles, and small-molecule conjugates.
- Collaborates with synthetic chemists and computational biologists both at the University and with third-party CROs to improve the affinity and physical properties of small molecules lead compounds.
- Translates knowledge of project-relevant scientific and patent literature into new therapeutic opportunities and research processes.
- Mentors and manages the research activities of post-docs, graduate students, research associates and undergraduate students to achieve research objectives.
- Serves as a resource for collecting data and performing analysis. Facilitates and promotes a research project by providing scientific or intellectual information.
- Develops laboratory protocols and training on new techniques. Manage, analyze and make recommendations on complex data sets for research.
- Creates first drafts for scientific writing and publications, including protocols and grants.
- Trains and mentors laboratory personnel.
- Performs other related work as needed.
Minimum requirements include a PhD in related field.---
Minimum requirements include knowledge and skills developed through 5-7 years of work experience in a related job discipline.---
- Ph.D. in chemistry or biology.
- Mammalian cell culture experience including transfection, viral transduction and CRISPR.
- Experience developing enzymatic and cell-based assays to study protein function and support small-molecule drug discovery.
- Experience in molecular biology methods including cloning, qPCR, RNAseq, and Western Blot.
- Experience in protein purification methods to support in vitro assay development, xray crystallography, and cryoEM.
Working ConditionsApplication Documents
- Have a quantitative mindset and a strong scientific intuition.
- Demonstrated ability to execute experiments, analyze data, and report conclusions.
- Must be flexible and willing to work across multiple projects.
- Ability to thrive in a highly collaborative, fast-paced, team-oriented environment with colleagues from diverse disciplines.
- Self-motivated and curious with a desire to learn from and teach others.
- Resume (required)
- Cover Letter (preferred)
When applying, the document(s) MUST
be uploaded via the My Experience
page, in the section titled Application Documents
of the application.Job Family
Individual ContributorFLSA Status
MonthlyScheduled Weekly Hours
YesRequires Compliance with University Covid-19 Vaccination Requirement
YesDrug Test Required
NoHealth Screen Required
YesMotor Vehicle Record Inquiry Required
Employees must comply with the University's COVID-19 vaccination requirements. More information about the requirements can be found on the University of Chicago Vaccination GoForward.
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Staff Job seekers in need of a reasonable accommodation to complete the application process should call 773-702-5800 or submit a request via Applicant Inquiry Form.
We seek a diverse pool of applicants who wish to join an academic community that places the highest value on rigorous inquiry and encourages a diversity of perspectives, experiences, groups of individuals, and ideas to inform and stimulate intellectual challenge, engagement, and exchange.
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